Methods to Investigate the Global Atmospheric Microbiome - ACL en SPI Accéder directement au contenu
Article Dans Une Revue Frontiers in Microbiology Année : 2019

Methods to Investigate the Global Atmospheric Microbiome

Pierre Amato
Romie Tignat-Perrier
  • Fonction : Auteur
  • PersonId : 1041451
Alban Thollot
  • Fonction : Auteur
Muriel Mourguy Joly
Karine Sellegri
  • Fonction : Auteur
  • PersonId : 842104
Jeroen E. Sonke
  • Fonction : Auteur
  • PersonId : 947595
Marcos Andrade
Lynwill Martin
  • Fonction : Auteur
Qianggong Zhang
  • Fonction : Auteur
Catherine Larose

Résumé

The interplay between microbes and atmospheric physical and chemical conditions is an open field of research that can only be fully addressed using multidisciplinary approaches. The lack of coordinated efforts to gather data at representative temporal and spatial scales limits aerobiology to help understand large scale patterns of global microbial biodiversity and its causal relationships with the environmental context. This paper presents the sampling strategy and analytical protocols developed in order to integrate different fields of research such as microbiology,-omics biology, atmospheric chemistry, physics and meteorology to characterize atmospheric microbial life. These include control of chemical and microbial contaminations from sampling to analysis and identification of experimental procedures for characterizing airborne microbial biodiversity and its functioning from the atmospheric samples collected at remote sites from low cell density environments. We used high-volume sampling strategy to address both chemical and microbial composition of the atmosphere, because it can help overcome low aerosol and microbial cell concentrations. To account for contaminations, exposed and unexposed control filters were processed along with the samples. We present a method that allows for the extraction of chemical and biological data from the same quartz filters. We tested different sampling times, extraction kits and methods to optimize DNA yield from filters. Based on our results, we recommend supplementary sterilization steps to reduce filter contamination induced by handling and transport. These include manipulation under laminar flow hoods and UV sterilization. In terms of DNA extraction, we recommend a vortex step and a heating step to reduce Frontiers in Microbiology | www.frontiersin.org 1

Mots clés

Fichier principal
Vignette du fichier
Dommergue etal2019, INHALEmethods.pdf (1.23 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02122072 , version 1 (06-11-2020)

Identifiants

Citer

Aurélien Dommergue, Pierre Amato, Romie Tignat-Perrier, Olivier Magand, Alban Thollot, et al.. Methods to Investigate the Global Atmospheric Microbiome. Frontiers in Microbiology, 2019, 10, pp.243. ⟨10.3389/fmicb.2019.00243⟩. ⟨hal-02122072⟩
142 Consultations
123 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More