Rapid chiral characterization of amino acids using non-covalent CuII complexes and TIMS-ToF separation
Résumé
Chiral molecules pose a unique challenge in life sciences, since enantiomers may exhibit different biological activity/toxicity while having similar physical and chemical properties.
Separation of enantiomers using mass spectrometry (MS) mostly relies on derivatization or formation of non-covalent complexes in the gas phase (with various metal/alkali cations and chiral selectors such as cyclodextrins or carbohydrates).
Ion-mobility spectrometry (IMS) separates ions based on their m/z ratio and their collision cross section (CCS), which makes it a promising tool for the rapid analysis of isomeric compounds. In addition, the resolving power of the TIMS-ToF instrument (Bruker Daltonics) used in this work can be increased (R ≈150-200) using single ion mobility monitoring (SIM2) and thus improve the mobility separation of stereoisomers.
Domaines
Chimie analytique| Origine | Fichiers produits par l'(les) auteur(s) |
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