High throughput separation of chiral molecules using non-covalent complexes and SIM2 analysis on a TIMS-ToF
Résumé
Chiral molecules pose a unique challenge in life sciences, since enantiomers may exhibit different biological activity/toxicity while having similar physical and chemical properties.
Separation of enantiomers using mass spectrometry (MS) mostly relies on derivatization or formation of non-covalent complexes (NCCs) in the gas phase (with various metal/alkali cations and chiral selectors such as cyclodextrins or carbohydrates). Alternatively, ion-mobility spectrometry (IMS) separates ions based on their m/z ratio and their collision cross section (CCS), which makes it a promising tool for the rapid analysis of isomeric compounds.
In this work, chiral analyses were conducted on a trapped ion mobility spectrometry time-of-flight (TIMS-ToF) instrument, using serial acquisitions by applying single ion mobility monitoring (SIM2) at high mobility resolving power and at different ion mobility ranges. The method was applied on various natural products such as amino acids (AAs) and biologically relevant molecules.
Domaines
Chimie analytique| Origine | Fichiers produits par l'(les) auteur(s) |
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