Development of a high-throughput and high ion mobility resolution approach using automatic stitching of multiple ion mobility ranges on a TIMS-ToF instrument: application to chiral analysis
Résumé
The characterization and identification of metabolites of interest in biological matrices is often achieved using hyphenated methods such as liquid chromatography coupled with mass spectrometry (LC-MS) but it remains an analytical challenge.
Ion mobility spectrometry is based on gas-phase mobility and its coupling with MS (IM‑MS) offers an additional separation dimension and may allow CCS determination of analytes without lengthening the acquisition time. High resolution conditions are nevertheless essential to distinguish ions with very close ion mobilities such as chiral compounds.
A high-throughput and high IM resolution approach was developed to allow exhaustive IM-MS measurements on a trapped ion mobility spectrometry time-of-flight (TIMS-ToF) instrument. Chiral amino acids (AAs) were chosen as model chiral compounds, and enantiomer couples were separated as non covalent complexes with CuII.
Domaines
Chimie analytique| Origine | Fichiers produits par l'(les) auteur(s) |
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