Assessment of the human corneal endothelium: in vivo Topcon SP2000P specular microscope versus ex vivo sambacornea eye bank analyser

Gilles Thuret 1 Nilanjana Deb-Joardar 1 Chloé Manissolle 1 Zhao Hui 1, 2 Michel Peoch 1 Philippe Gain 1 Yann Gavet 3, 4, 5, 6
1 SAINTOPH - Service d'ophtalmologie [CHU de Saint-Etienne]
2 LFMSCMS - Laboratory of Functional Materials, School of Chemistry and Materials Science
3 IFRESIS-ENSMSE - Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé
4 CIS-ENSMSE - Centre Ingénierie et Santé
5 DIS-ENSMSE - Département Imagerie et Statistiques
6 LPMG-EMSE - Laboratoire des Procédés en Milieux Granulaires
Abstract : Comparison between assessment of donor tissue in eye banks and specular microscopy in the recipient is important to quantify the post-keratoplasty cell loss dynamics. Our aim was to determine the agreement between the in vivo non-contact specular microscope Topcon SP2000P and the computer-assisted eye bank endothelial analyser Sambacornea. We enrolled 51 future recipients of penetrating keratoplasty, and determined the endothelial cell density (ECD) and morphometry firstly in vivo with Topcon and then ex vivo with Sambacornea on the excised cornea stained with Alizarin Red. Specular microscopy was found to underestimate the ECD by 11%, (95% CI 6 to 15), whereas morphometric parameters did not differ.
Keywords : human corneal endothelium Cell-density image-analysis donor corneas Topcon SP2000P specular microscope post-keratoplasty cell loss dynamics eye bank endothelial analyser Sambacornea endothelial cell density (ECD)
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Article dans une revue
British Journal of Ophthalmology, BMJ Publishing Group, 2007, 91 (2), pp.265-266. 〈10.1136/bjo.2006.099614〉
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https://hal-emse.ccsd.cnrs.fr/emse-00446096
Contributeur : Andrée-Aimée Toucas <>
Soumis le : mardi 12 janvier 2010 - 09:31:24
Dernière modification le : lundi 23 avril 2018 - 10:40:06

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Gilles Thuret, Nilanjana Deb-Joardar, Chloé Manissolle, Zhao Hui, Michel Peoch, et al.. Assessment of the human corneal endothelium: in vivo Topcon SP2000P specular microscope versus ex vivo sambacornea eye bank analyser. British Journal of Ophthalmology, BMJ Publishing Group, 2007, 91 (2), pp.265-266. 〈10.1136/bjo.2006.099614〉. 〈emse-00446096〉

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