Enumeration of peripheral lymphocyte subsets using 6 vs. 4 color staining: A clinical evaluation of a new flowcytometer

Abstract : The day-to-day instrument detection but automatic compensations were stable. Manual compensation settings were satisfactory using available facilities. Commercial and UK NEQAS quality control results were acceptable. The intra-experiment reproducibility was good (coefficient of variation (CV) <3%) but highly operator-dependant (CD4+ T cell count CVs from 1.2 to 9.7, six operators). Storage of samples was acceptable, but storage of stained samples altered absolute count reliability. Serial dilutions show a good count accuracy. The FACScanto T subsets and B cell data were highly correlated with our reference values (r2 > 0.87) and absolutes values were very close (slopes > 0.89). The gating strategy, fluorochrome choice, and compensation setting are discussed. A few improvements are expected (sample loader, data management, auto-gating, acquisition parameters, sample mixing, absolute values calculation, etc). In conclusions, despite its complexity, 6 color staining is a reliable, stable, and highly informative technique for lymphocyte subset monitoring but remains to be optimized.
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Cytometry Part B : Clinical Cytometry, 2006, 70B (1), pp.29-38. 〈10.1002/cyto.b.20072〉
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https://hal-emse.ccsd.cnrs.fr/emse-00497885
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Soumis le : mardi 6 juillet 2010 - 10:24:34
Dernière modification le : mardi 23 octobre 2018 - 14:36:09

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Claude Lambert, Cristina Iobagiu, Christian Genin. Enumeration of peripheral lymphocyte subsets using 6 vs. 4 color staining: A clinical evaluation of a new flowcytometer. Cytometry Part B : Clinical Cytometry, 2006, 70B (1), pp.29-38. 〈10.1002/cyto.b.20072〉. 〈emse-00497885〉

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