In this work, the application of the unnotched Iosipescu test for the evaluation of the radial variability of longitudinal–radial stiffness parameters of maritime pine wood was investigated. Rectangular specimens with grain at 45° were tested using the Iosipescu fixture. For this configuration both the transverse (Q22) and the shear (Q66) stiffness parameters can be simultaneously identified by the virtual fields method. Displacement fields were measured by the grid method. The strain fields were reconstructed from the raw displacement fields using a polynomial approximation scheme. For the tested wood material, it was found that both parameters decrease from the centre to about the middle radius of the stem and increase afterwards to the outermost positions. Moreover, a relatively good correlation was obtained between the patterns of radial variability of Q22 and Q66.