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Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support

Abstract : An amperometric enzyme sensor using tyrosinase, also called polyphenol oxidase (PPO), was constructed for determination of phenolic compounds and herbicides. The enzyme was entrapped in a conducting polymer, poly 3,4-ethylenedioxythiophene (PEDT), electrochemically generated on a glassy carbon electrode. Several experimental parameters in the electropolymerisation process and working conditions were determined to optimise biosensor performances. Mono-phenol and di-phenol were tested in oxygenated solutions, by amperometric measurements at −200 mV (vs. SCE) in a batch system. The limit of detection of these molecules ranges from 5 to 500 nM. Detection of herbicides was obtained from the inhibition of tyrosinase electrode responses. The limit of detection for atrazine and diuron was 1 and 0.5 mg l−1 respectively. These data suggest that PEDT film is a promising PPO immobilisation method.
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Christophe Védrine, Silvia Fabiano, Canh Tran-Minh. Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support. Talanta, Elsevier, 2003, 59 (3), pp.535-544. ⟨10.1016/S0039-9140(02)00540-4⟩. ⟨emse-00520263⟩

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