Application and automation of flow injection analysis (FIA) using fast responding enzyme glass electrodes to detect penicillin in fermentation broth and urea in human serum - Mines Saint-Étienne Access content directly
Journal Articles Journal of Automatic Chemistry Year : 1992

Application and automation of flow injection analysis (FIA) using fast responding enzyme glass electrodes to detect penicillin in fermentation broth and urea in human serum

Abstract

An enzyme immobilization technique has been developed to determine the concentration of biological compounds. This technique has been applied to penicillinase and urease, which are crosslinked as very fine films directly onto the sensitive ends of pH glass electrodes, thereby dispensing with the need of an on-line enzyme reactor. The biosensor is incorporated in an FIA system within a magnetically stirred detection cell. Penicillin-V in fermentation broth and urea in human serum samples were detected and the results were compared with HPLC and spectrophotometric methods. On-line measurement is achieved through the automation of this FIA system.

Dates and versions

emse-00567402 , version 1 (21-02-2011)

Identifiers

Cite

Helmut Meier, François Lantreibecq, Canh Tran Minh. Application and automation of flow injection analysis (FIA) using fast responding enzyme glass electrodes to detect penicillin in fermentation broth and urea in human serum. Journal of Automatic Chemistry, 1992, 14 (4), pp.137-143. ⟨10.1155/S1463924692000269⟩. ⟨emse-00567402⟩
20 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More