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Journal Articles Analytica Chimica Acta Year : 1991

Rapid measurement of penicillin contained in complex media using enzyme-loaded glass electrodes

Abstract

An enzyme electrode based on a glass electrode for measuring penicillin-V concentration is described. In preparing this electrode, penicillinase is immobilized by cross-linking with a very fine film of glutaraldehyde, which is sprayed directly over the sensitive end of a pH glass electrode. The penicillin sensor was used for the determination of penicillin-V concentrations in phosphate buffer, fermentation broth and dairy milk, the ranges of concentrations detected being 1.5-15, 2.5-15 and 2-12 mM, respectively. The linearity, stability, sensitivity, reversibility and pH and buffer strength dependence of the electrode were examined and are discussed. An extremely short response time of less than 10 s is obtained with electrodes made in this manner.

Dates and versions

emse-00567925 , version 1 (22-02-2011)

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Helmut Meier, Satish Kumaran, Anne-Marie Danna, Canh Tran Minh. Rapid measurement of penicillin contained in complex media using enzyme-loaded glass electrodes. Analytica Chimica Acta, 1991, 249 (2), pp.405-411. ⟨10.1016/S0003-2670(00)83013-5⟩. ⟨emse-00567925⟩
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