Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase

Abstract : Acetylcholine esterase electrodes, based on glass, Pd/PdO and Ir/IrO2 electrodes as pH sensor, using the immobilized acetylcholine esterase in acrylamide-methacrylamide hydrazides prepolymer are reported and compared. New data on the analysis of nicotine, fluoride ion, and some organophosphorus compounds are reported using the present AChE sensor based on the inhibition of the immobilized acetylcholine esterase. Reactivation of immobilized AChE after inhibition with reversible inhibitor, i.e. nicotine and fluoride ion is carried out using a mixture of working buffer and acetylcholine, whereas reactivation after inhibition with irreversible inhibitor, i.e. organophosphorus compounds is carried out using a mixture of acetylcholine and pyridine-2-aldoxime methiodide (PAM). The detection limits for the nicotine and fluoride ion are found to be 10−5M whereas for paraoxon, methyl parathion and malathion are found to be 10−9 M and 10−10 M.
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Biosensors and Bioelectronics, Elsevier, 1990, 5 (6), pp.461-471. 〈10.1016/0956-5663(90)80035-C〉
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Soumis le : jeudi 24 février 2011 - 17:10:36
Dernière modification le : lundi 25 juillet 2011 - 14:40:35

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Canh Tran Minh, P.C. Pandey, Satish Kumaran. Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase. Biosensors and Bioelectronics, Elsevier, 1990, 5 (6), pp.461-471. 〈10.1016/0956-5663(90)80035-C〉. 〈emse-00569329〉

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