Corporate social responsibility (CSR) is receiving an increased attention in biodiversity policy. This exploratory study analyses biodiversity-oriented corporate practices and stakeholders’ involvement in their implementation based on a content analysis of 34 business commitment plans endorsed as contributions to the French National Biodiversity Strategy and Action Plan (NBSAP). A trans-sectoral typology of practices emerged from this analysis. This typology categorizes, on one hand, CSR practices that directly mitigate biodiversity loss, and on the other hand, procedural activities supporting a better integration of biodiversity issues. External stakeholders were found to be involved in more than two thirds of the CSR actions and played different roles depending if they belonged to the territorial system, the value chain or the institutional system the company. The article concludes with a number of implications for policy-makers as well as business managers and opens research avenues.