The application of new technics of enzyme insolubilization has made it possible to bind enzymes inside artificial membranes structures, thus opening up opportunities for a better knowledge of the biologic function of membranes and the therapeutic use of new membranes possessing properties comparable to those of biologic membranes. These new possibilities result from the availability of new chemical technics that enable the permanent binding of enzymes on insoluble carriers without destroying their activity. Some binding methods recently have been applied to a large number of different enzymes, especially employing bifunctional agents such as bi-diazotized benzidine, bi-diazotized o. dianisidine, glutaraldehyde, chlorotriazines and so forth...