Synthesis of benzoates by enzymatic catalysis in heterogenous medium.

Abstract : The enzyme-catalyzed synthesis of methyl and 1-butyl benzoates is reported. Direct esterification is performed with good yields by lipase from Candida rugosa suspended in a hexane/toluene mixture. Influence of substrates concentration, water content and toluene ratio are examined. Methanol and 1-butanol are lipase-inhibitors: they interact with the water adsorbed on lipase and on the enzyme as well. Increasing benzoic acid concentration in the system partially eliminates the alcohol inhibition. Water content greatly affects reaction kinetics and must be optimized to both activate the lipase and reach good conversion yields. Toluene, used to solubilize benzoic acid, negatively acts on reaction kinetics, mostly by increasing the partition of benzoic acid for the organic phase. The kinetic modelisation of 1-butyl benzoate synthesis agrees with a ping-pong Bi–Bi mechanism with 1-butanol inhibition. Apparent reaction coefficients are then determined.
Type de document :
Article dans une revue
Journal of Molecular Catalysis B: Enzymatic, Elsevier, 1998, 5 (1-4), pp.277-281
Liste complète des métadonnées

https://hal-emse.ccsd.cnrs.fr/emse-00494419
Contributeur : Andrée-Aimée Toucas <>
Soumis le : mercredi 23 juin 2010 - 10:55:23
Dernière modification le : jeudi 3 février 2011 - 10:34:40

Identifiants

  • HAL Id : emse-00494419, version 1

Collections

Citation

Jean-Pierre Leszczak, Canh Tran-Minh. Synthesis of benzoates by enzymatic catalysis in heterogenous medium.. Journal of Molecular Catalysis B: Enzymatic, Elsevier, 1998, 5 (1-4), pp.277-281. 〈emse-00494419〉

Partager

Métriques

Consultations de la notice

27