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Unease-gelatin interdigitated microelectrodes for the conductometric determination of protease activity

Abstract : Conductometric microbiosensors for the determination of trypsin were elaborated via the modification of microfabricated interdigitated gold electrodes by a cross-linked urease/BSA coating covered by a gelatin film. The resulting microelectrodes were exposed to different trypsin concentrations ranging from 100 pg/mL to 1 mg/mL (1 mU/mL to 10,000 U/mL) for selective proteolytic degradation of the gelatin film. Then, the conductometric response of the microbiosensors to urea (33 mu M) was recorded as a function of the trypsin concentration, the gelatin amount (8-80 ng) and the incubation time (40 s, 100 min). The optimum incubation time for each trypsin concentration was determined leading to a detection limit of 100 pg/mL (1 mU/mL). In these optimized conditions, the proof of concept of this sensitive, disposable, low-cost and label-free trypsin biosensors based on a conductometric transducer was demonstrated for the first time. (C) 2008 Elsevier B.V. All rights reserved.
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https://hal-emse.ccsd.cnrs.fr/emse-00502030
Contributor : Anna Fraczkiewicz <>
Submitted on : Tuesday, July 13, 2010 - 10:07:10 AM
Last modification on : Friday, July 17, 2020 - 8:34:02 AM

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Rodica Elena Ionescu, Chrystelle Fillit, Nicole Jaffrezic-Renault, Serge Cosnier. Unease-gelatin interdigitated microelectrodes for the conductometric determination of protease activity. Biosensors & bioelectronics, 2008, 24 (3), pp.489-492. ⟨10.1016/j.bios.2008.06.021⟩. ⟨emse-00502030⟩

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