Development of innovative pH sensor to evaluate phagocytosis of nanoparticles
Abstract
Inhaled nanoparticles (NP) exhibit variable toxicity levels which mainly depend on their physicochemical characteristics (size, morphology, crystallinity, chemical surface composition...). Biological effects monitoring thanks to usual tests (ROS, TNFα, LDH) are performed on alveolar macrophages collected from the respiratory system. In this frame, evaluation of NP uptake by macrophages appears as complementary data useful for NP toxicity assessment. The aim of this work deals with the development of pH sensible NP allowing the quantification of NP phagocytosed by macrophages, specially the step of fusion between phagosomes and lysosomes.
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