The extreme heterogeneity and interconnectivity of the 3-dimensional pore space within soil makes it a unique habitat for the diverse microbial population and has a pivotal role in microbial interactions. Manipulation and quantification of the 3-dimensional pore space and the spatial distribution of micro-organisms is therefore essential if we are to fully understand microbial interactions. Here we pack soil microcosms at different bulk-densities to manipulate soil structure and use x-ray micro tomography and soil thin sections to analyse the effect on the connectivity of the pore volume and on fungal exploration.