Universities face a paradigmatic educational challenge, driven by Industry 4.0 technologies such as Cloud Computing, producing the need to develop new teaching competencies. The question arises: How can we diagnose the level of Cloud Computing competencies that teachers have in the context of Education 4.0? To address this question, the objective was to design a valid and reliable instrument to measure these competencies. A literature review and expert collaboration were carried out. For validation, it was submitted to expert judgment and Kendall's W concordance coefficient was calculated; for reliability, a pilot test was carried out with teachers of the Computer Systems Engineering career and the KR20 (Kuder-Richardson) was used. The result was an instrument with 23 items capable of measuring seven competencies identified by the collaborative network Industry 4.0 Node. This instrument contributes as a guideline for educational institutions, bridging the gap between technological progress and formal education.